Cryopreservation of Parathyroid Tissue

Parathyroid tissue to be cryopreserved is confirmed to be parathyroid tissue by frozen section examination, and a portion is immediately placed on ice in physiologic saline. The tissue is subsequently placed into a Petri dish in RPMI-1640 tissue culture medium. The cold parathyroid tissue is sectioned into 1- to 2-mm pieces with a scalpel blade. Using sterile technique, about five to eight pieces are placed into a cryovial, and several vials are usually prepared. We then add 0.5 mL of 20% dimethyl sulfoxide in RPMI-1640 and 0.5 mL of RPMI-1640 containing 20% of serum from the patient per vial. The tissue is transferred first to the -80° C freezer to freeze slowly (temperature decreases about 1° C per minute); then it is transferred to be stored in liquid nitrogen. Complete patient information and location are documented in case the tissue is subsequently needed to treat hypoparathyroidism.

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