Free Radical Scavenging Assay

This spectrophotometric assay uses the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) as a reagent (Yadegarinia et al., 2006). The model of scavenging stable DPPH-free radicals can be used to evaluate the antioxidative activities in a relatively short time (Conforti et al., 2006). The samples are able to reduce the stable free DPPH radical to 1,1-diphenyl-2-picrylhydrazyl that is yellow colored. The hydrogen or electron donation abilities of the samples are measured by means of the decrease of the absorbance resulting in a color change from purple to yellow (Gutierrez et al., 2006). Another procedure can be applied for an EO. A dilution of the EO in toluene is applied onto a thin-layer chromatography (TLC) plate and toluene-ethyl-acetate is used as a developer (Sokmen et al., 2004a). The plates are sprayed with 0.4 mM DPPH in methanol. The active compounds were detected as yellow spots on a purple background. Only those compounds, which changed the color within 30 min, are taken as a positive result.

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Aromatherapy Ambiance

Aromatherapy Ambiance

Aromatherapy, a word often associated with calm, sweet smelling and relaxing surroundings. Made famous for its mostly relaxing indulgent  feature, using aromatherapy has also been known to be related to have medicinal qualities.

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