Determination of the Seminal Antioxidant Capacity

The antioxidant activity in biological fluids can be measured either by determination of the concentrations of the individual antioxidants or by the TAC [168, 169]. Among the different methodologies, which include the determination of the oxygen radical absorbance capacity [170], ferric reducing ability [171] or the phycoerythrin fluorescence-based assay [172] . an enhanced chemiluminescent assay described by Whitehead et al. [173] is most commonly used. The principle of this assay is that the chemiluminescence emitted by a chemiluminescent substance such as luminol is inhibited by the antioxidant activity and then compared and standardized with that of Trolox® (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), a water-soluble vitamin E analogue, and measured as molar Trolox® equivalent. More recently, a reliable, accurate, more rapid and simple colorimetric method has been developed [174],

The determination of TAC in seminal plasma revealed significant differences between fertile and infertile subjects with TAC being higher in fertile men [168, 175, 176]. Said et al. [174] developed a colorimetric assay, which is simple, relatively cheap and easy to perform, and was as reliable as the luminometric test system. More recently, Mahfouz et al. [177] have established a clinical cut-off value for the TAC of 1,420 |M and thus demonstrated its diagnostic value. Yet, proper clinical evaluation including the calculation of cut-off values by means of ROC still has to be carried out.

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