Determination of the Seminal Antioxidant Capacity

The antioxidant activity in biological fluids can be measured either by determination of the concentrations of the individual antioxidants or by the TAC [168, 169]. Among the different methodologies, which include the determination of the oxygen radical absorbance capacity [170], ferric reducing ability [171] or the phycoerythrin fluorescence-based assay [172] . an enhanced chemiluminescent assay described by Whitehead et al. [173] is most commonly used. The principle of this assay is that the chemiluminescence emitted by a chemiluminescent substance such as luminol is inhibited by the antioxidant activity and then compared and standardized with that of Trolox® (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), a water-soluble vitamin E analogue, and measured as molar Trolox® equivalent. More recently, a reliable, accurate, more rapid and simple colorimetric method has been developed [174],

The determination of TAC in seminal plasma revealed significant differences between fertile and infertile subjects with TAC being higher in fertile men [168, 175, 176]. Said et al. [174] developed a colorimetric assay, which is simple, relatively cheap and easy to perform, and was as reliable as the luminometric test system. More recently, Mahfouz et al. [177] have established a clinical cut-off value for the TAC of 1,420 |M and thus demonstrated its diagnostic value. Yet, proper clinical evaluation including the calculation of cut-off values by means of ROC still has to be carried out.

Delicious Diabetic Recipes

Delicious Diabetic Recipes

This brilliant guide will teach you how to cook all those delicious recipes for people who have diabetes.

Get My Free Ebook

Post a comment