Diagnostic Application of Chemiluminescence Assays

Chemiluminescent assays provide clinically significant results that correlate with the quality of the ejaculate and the fertilizing potential of the spermatozoa both in vitro and in vivo. This is especially true in patients presenting with poor sperm quality such as in cases of oligozoospermia, or in samples where low-density immature spermatozoa recovered following sperm preparation using a density gradient technique. Defective sperm populations such as those exhibiting presence of excess residual cytoplasmic are associated with high redox activity [6, 13, 36-38] . This assay can also be used as an important diagnostic screening tool in the evaluating patients with unexplained or idiopathic infertility as these patients demonstrate high ROS levels in their seminal ejaculates.

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