Luminometers

Several types of luminometers, varying ranging in features, design, and pricing, can be used in measuring the emitted light from the chemiluminescence assay reaction (Table 13.4) [32]. The measurements from the chemiluminescence assay are expressed as either counted photons per minute (cpm) or as relative light units (RLU). There are two different kinds of processing designs for luminometers. While direct current luminometers measure electric current, photon counting luminometers count individual photons [ 33] . Moreover, there are currently three types of luminometers available for commercial uses. First is single and double tube luminometers, which measure one or two samples at a time. These are inexpensive and are typically used by small research laboratories. Secondly, multiple tube luminom-eters that measure several tubes at a time. They are more expensive than single and double tube luminometers and are used by research centers that depend on chemilu-minescence for research projects. Lastly, plate luminometers measure multiple tubes at a given time and are typically used by core research laboratories and commercial enterprises.

Table 13.4 Commercially available luminometers shown by type, sensitivity, and manufacturer

Model

Type

Sensitivity and dynamic range

Manufacturer

GloMax 20/20

Single tube

0.1 fg luciferase

Promega Corporation

(Sunnyvale, CA, USA)

FB-12

Single tube

1,000 molecules of luciferase

Zylux Corporation (Oak

Ridge, TN, USA)

Triathler

Single tube

<10 amol ATP/vial

Hidex (Turku, Finland)

Optocomp-2

Multiple tube

0.1 pg ATP

MGM Instruments

(Hamden, CT, USA)

Autolumat Auto

Multiple tube

5 amol of ATP

Berthold Technologies

Plus LB 953

(Oak Ridge, TN, USA)

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