Methods for Detecting ROS

There are several techniques to detect ROS. These include the direct and indirect methods (Table 13.2). Direct methods include cytochrome c reduction, electron spin resonance, and nitroblue tetrazolium technique (NBT, and xyenol orange-based assay and flow cytometry [7, 22]). Indirect methods of measurement include the Endtz test, redox potential (GSH/GSSG), measurement of lipid peroxidation products levels, chemokines, measurement of oxidative DNA damage, and measurement of reactive nitrogen species by Greiss reaction and fluorescence spectroscopy [7] .

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