Methods for the Detection of ROS in Human Sperm Samples

David Benjamin, Rakesh K. Sharma, Arozia Moazzam, and Ashok Agarwal

Abstract Male-factor significantly contributes to infertility in couples of reproductive age. Several studies have shown that oxidative stress (OS) is involved in the pathophysiology of male infertility. It is caused by an imbalance between the formation of reactive oxygen species (ROS) and the ability of the antioxidants to scavenge them. There are several methods to measure seminal ROS in the clinical setting, most notably among them is the chemiluminescence assay. This technique measures the global ROS, i.e., both the intra- and extracellular ROS. The measurement of seminal ROS employing the chemiluminescence technique in clinical andrology labs is explained in this chapter. Through a deeper understanding of ROS and its measurement, clinical andrology labs can better assist patients to achieve increased rates of fertility and pregnancy.

Keywords Use of chemiluminescence assay • Measurement of seminal reactive oxygen levels • Clinical andrology labs • Free radicals • Sources of ROS

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