Sperm Preparation Techniques

Sperm preparation techniques can be used to separate ROS producing agents such as leukocytes and immature spermatozoa from mature spermatozoa. The use of density gradients and centrifugation separates mature and immature sperm populations based on morphology and motility [88] . Swim up techniques may also be used to separate highly motile and morphologically normal sperm from the rest of the sperm population [89]. Percoll density gradient preparation is available, but is recommended for research purposes only.

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