Single Strand Conformation Polymorphism Analysis of Mutations in Exons 48 of the TP53 Gene

Vahakangas, Katariina Castren, and Judith A. Welsh 1. Introduction The TP53 tumor suppressor gene coding for a nuclear phosphoprotein involved in cellular stress responses is the most frequently mutated gene in human cancers described so far (1-4). Mutations are found throughout the gene but most frequently within the highly conserved middle region (exons 5-8) that encodes for the DNA-binding central region of the gene critical for the major function of TP53 protein as a...

James G Donnelly 1 Introduction

Deep vein thrombosis (DVT) can be the result of coagulation pathway defects at the molecular level or damage to the vascular endothelium. Some of the acquired causes of DVT include malignancy, trauma, prolonged immobilization, and pregnancy (1). Thrombophilia can be owing in part to both acquired and inherited defects. The relative risk for thrombosis is increased by estrogen replacement therapy (2) and homocysteinemia (3). Homocysteine metabolism is influenced by the use of alcohol,...

Detection of Hcv Rna Using the Amplicor Hcv Test Version

The AMPLICOR HCV test is for use only with serum or plasma specimens. For serum samples, collect blood in VACUTAINER serum separator tubes (Becton-Dickinson no. 367784 or 367789), and for plasma, collect blood in tubes containing EDTA (lavender top, Becton-Dickinson no. 6454 or equivalent) or acid citrate dextrose (yellow top, Becton Dickinson no. 4606 or equivalent) as the anticoagulant. Specimens collected in tubes containing heparin as the anticoagulant are unsuitable for this test. 2.1.2....

Detection of Microsatellite Instability

Griffin, and James R. Eshleman 1. Introduction In 1993, three groups independently discovered that the lengths of microsatellites in tumors could vary from the normally constant pattern defined at birth (1-3) (see review in ref. 4). This discovery has been designated either microsatellite instability (MSI) or replication errors (RER). A recent international consensus conference convened by the National Cancer Institute defined MSI RER as a change in length due to...