Info

Percent Block

[B]

Ton

at Equilibrium

(MM)

(sec)

(%)

7.5

0.4

62

20

0.2

83

45

0.1

95

b. Calculate the microscopic rate constants k+B and k_B and the equilibrium constant KB. Using these and the equation you derived in part (a), calculate the percent block expected at equilibrium for each of the antagonist concentrations used. How well do these calculated values agree with those observed experimentally? Suggest possible reasons why the calculated equilibrium block might not agree with that observed experimentally. Describe what a single-channel recording of the receptor activity would look like at equilibrium in the presence of the agonist alone and in the presence of agonist plus antagonist.

Was this article helpful?

0 0

Post a comment