M Simoni

Contents

21.1

Introduction

21.2

Testosterone in blood

21.3

Principles of immunological testosterone assays

21.4

Measurement oftestosterone

21.4.1

Isotope dilution-mass spectrometry

21.4.2

Radioimmunoassay

21.4.3

Other immunoassays

21.4.4

Assessment of free testosterone

21.4.4.1

Equilibrium dialysis

21.4.4.2

Ultrafiltration

21.4.4.3

Direct free testosterone RIA

21.4.4.4

Salivary testosterone

21.4.4.5

Bioavailable testosterone

21.4.4.6

Calculated free testosterone

21.4.5

Bioassay

21.5

Measurement of DHT

21.6

Quality control

21.6.1

Choice of the kit and assay validation

21.6.2

Internal quality control

21.6.3

External quality assessment

21.7

Key messages

21.8

References

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