Principles of immunological testosterone assays

The principles of hormone measurement in general also apply to testosterone and good chapters on hormone assays are available in various textbooks of endocrinology (e.g. Segre and Brown 1998). As for all other hormones, the accurate measurement of testosterone in blood was made possible by the advent of radioimmunoassays (RIA). In immunological assays the hormone being measured (i.e. the antigen) competes with the labeled hormone (i.e. the tracer) forbindingto an antiserum (the antibody). Since the amount of antibody available for reaction is limited, the higher the concentration of the hormone in blood, the lower the amount of tracer bound

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